Lead Criminalist Horatio Caine is targeted by adversaries old and new. As the danger grows for Caine, he and the rest of his team - Calliegh, Eric, Alexx, Ryan, Frank and Natalia - must go the limit to solve their biggest cases yet. The team collects and analyzes the evidence to solve crimes and vindicate the victims.

Title from disc 1 label. Originally broadcast on CBS during the 2006-2007 season. Contains all 24 episodes from the fifth season ; episodes appear in order of their original air dates. Special features: Disc 1: Episode 501 with audio commentary by Sam Hill, Sunil Nayar and Brian O'Neill; Episode 503 with audio commentary by Sam Hill, Adam Rodriguez and Carey Meyer; "Rio Grand: CSI: Miami goes to Rio" featurette ; Disc 2: Episode 505 with audio commentary by Jonathan Togo, Corey Miller and Larry Detwiler; episode 507 with audio commentary by Eva La Rue and John Haynes; "If looks could kill: special F/X makeup" featurette. Special features: Disc 4: Episode 515 with audio commentary by Karen Gaviola and Gonzalo Menendez; "Turning up the heat: the look of CSI: Miami" featurette ; Disc 5: "Dressing the part" featurette ; Disc 6: "The real MDPD with Jonathan Togo" featurette ; PC CD-ROM playable demo "CSI: Crime Scene Investigation: Hard Evidence" (Rated: M for Mature - 17+; blood and gore, sexual themes, violence).

Disc 1 : Rio -- Going under -- Death pool 100 -- If looks could kill ; Disc 2 : Death Eminent -- Curse of the coffin -- High octane -- Darkroom ; Disc 3 : Going, going, gone -- Come as you are -- Backstabbers -- Internal affairs ; Disc 4 : Throwing heat -- No man's land -- Man down -- Broken home ; Disc 5 : A grizzly murder -- Triple threat -- Bloodline -- Rush ; Disc 6 : Just murdered -- Burned -- Kill switch -- Born to kill.